Research Article

Differential Proteome Profiling Using iTRAQ in Microalbuminuric and Normoalbuminuric Type 2 Diabetic Patients

Table 2

Selected 10 differentially excreted proteins related to pathogenic status in microalbumiuric versus normoalbuminuric urines.

Pathogenic status Number of unique peptidesaAccession numberbGene namecMA : NA expression
iTRAQd2-DEeWBf

Glomerular dysfunction115spt∣P02787TF1.86
247Spt∣P00450CP2.09
3209spt∣P01009A1AT1.42
449spt∣P00738HP2.35
510trm∣Q9UMV3MASP20.29
6235spt∣P98160HSPG0.68

Tubular dysfunction79spt∣P02774GC, VDBP 2.44
8414spt∣P02763ORM1, AGP1 2.04

Other types of diseases99spt∣Q01469FABP0.29
105trm∣O43653PSCA1.70

aThe numbers of unique peptides and MS/MS spectrum observed by ProteinPilot software were determined only for those peptides with ≥95% confidence. bAccession numbers represent entries in the Human CDS database (human KBMS 5.0, 2005-03-02; a total of 187,748 entries provided by Applied Biosystems). cGene name from the Expasy database correspond to protein accession number bfrom the Human CDS database (human KBMS 5.0, 2005-03-02; a total of 187,748 entries provided by Applied Biosystems). d–fRatio of differentially excreted protein for iTRAQ, 2-DE and WB in microalbumiuric versus normoalbuminuric urines, respectively.