Differential Proteome Profiling Using iTRAQ in Microalbuminuric and Normoalbuminuric Type 2 Diabetic Patients
Table 4
Parameters of MRM Experiment for seven candidate proteins.
Protein name
Q1a
Q3b
Sequencec
Fragmentd
Chargee
CEf
Transferrin
482.8
682.4
APNHAVVTR
y6
2+
26
315.2
558.3
AVGNLR
y5
2+
19
315.2
459.3
AVGNLR
y4
2+
19
Ceruloplasmin
686.4
1080.0
GAYPLSIEPIGVR
y10
2+
35
686.39
870.5
GAYPLSIEPIGVR
y8
2+
35
Alpha-1-antitrypsin
555.81
997.5
LSITGTYDLK
y9
2+
29
555.81
797.4
LSITGTYDLK
y7
2+
29
393.2
587.3
VVNPTQK
y5
2+
22
393.2
473.3
VVNPTQK
y5
2+
22
Haptoglobin precursor
729.8
1084.5
NLFLNHSENATAK
y10
2+
37
352.2
517.3
VSVNER
y4
2+
20
Vitamin D-binding protein
400.2
700.4
VLEPTLK
y6
2+
26
400.2
587.3
VLEPTLK
y5
2+
26
Alpha-1-acid glycoprotein 1
556.8
811.4
SDVVYTDWK
y6
2+
29
580.8
974.5
WFYIASAFR
y8
2+
31
580.8
827.4
WFYIASAFR
y7
2+
31
Prostate stem cell antigen
501.0
830.5
AVGLLTVISK
y8
2+
30
501.0
660.4
AVGLLTVISK
y6
2+
30
a-bQ1 and Q3 (m/z) represent the Q1 and Q3 transitions for proteotypic peptide, respectively. cSequence represents the sequence of proteotypic peptide for target protein. dFragment type indicates the ion type of the Q3 transition. eCharge represents the charge state of precursor ion. fCE represents collision energy.
