Differential Proteome Profiling Using iTRAQ in Microalbuminuric and Normoalbuminuric Type 2 Diabetic Patients
Table 5
Differentially excreted urinary proteome in microalbumiuric versus normoalbuminuric urine.
Unique peptidesa
Accession numberb
Protein name
Ratioc MA : NA
Pvald MA : NA
EFe MA : NA
1
651
spt∣P02768
Serum albumin
3.09
0.00
1.04
2
337
gb∣AAF01333.1
Serum albumin
0.36
0.00
1.08
3
669
trm∣Q8N4N0
Alpha-2-glycoprotein 1
1.48
0.00
1.02
4
639
rf∣NP_003352.1
uromodulin
0.72
0.00
1.04
5
269
emb∣CAA42438.1
Zn-alpha2-glycoprotein
1.80
0.00
1.06
6
235
spt∣P98160
HSPG
0.68
0.00
1.04
7
209
spt∣P01009
Alpha-1-antitrypsin
1.42
0.00
1.04
8
414
spt∣P02763
Alpha-1-acid glycoprotein 1
2.04
0.00
1.03
9
265
spt∣P02788
Serotransferrin
2.46
0.00
1.10
10
46
spt∣P02760
AMBP protein
1.44
0.00
1.12
11
300
spt∣P07911
Uromodulin
0.24
0.00
1.08
12
106
prf∣765044A
Ig G1 H Nie
0.61
0.00
1.15
13
223
dbj∣BAC85395.1
Unnamed protein product
1.37
0.00
1.10
14
217
emb∣CAA29229.1
Alpha-1-acid glycoprotein 1
2.29
0.00
1.11
15
107
trm∣Q5VU27
Heparan sulfate proteoglycan 2
2.00
0.00
1.18
16
306
spt∣P07998
Ribonuclease pancreatic
0.80
0.00
1.08
17
62
spt∣P41222
Prostaglandin-H2 D-isomerase
1.40
0.00
1.13
18
47
spt∣P00450
Ceruloplasmin
2.09
0.00
1.12
19
117
prf∣763134A
Ig A1 Bur
1.60
0.02
1.39
20
46
cra∣hCP1909255
Serine proteinase inhibitor
1.52
0.00
1.07
21
90
spt∣P10451
Osteopontin
0.57
0.00
1.43
22
9
spt∣P04746
Pancreatic alpha-amylase
0.41
0.00
1.33
23
39
spt∣P02749
Beta-2-glycoprotein I
1.37
0.00
1.07
24
50
trm∣Q9UII8
E-cadherin
1.36
0.00
1.12
25
34
rf∣NP_006112.2
Keratin 1
0.60
0.00
1.16
26
88
spt∣Q14624
ITIH4
0.78
0.00
1.08
27
21
trm∣Q6N025
FN
1.27
0.01
1.20
28
21
trm∣Q8N175
Keratin 10
0.67
0.00
1.27
29
34
emb∣CAA48671.1
Alpha1-antichymotrypsin
1.64
0.00
1.17
30
50
spt∣P00738
Haptoglobin
2.36
0.01
1.24
31
22
gb∣AAA52014.1
Cholesterol esterase
0.46
0.00
1.10
32
63
spt∣P05451
Lithostathine 1 alpha
1.54
0.00
1.05
33
31
trm∣Q6PAU9
Kininogen 1
0.75
0.00
1.18
34
20
spt∣P04217
Alpha-1B-glycoprotein
1.86
0.00
1.31
35
34
spt∣P05155
Plasma protease C1 inhibitor
0.75
0.00
1.12
36
14
trm∣Q8N473
Alpha 1 type I collagen
0.72
0.00
1.14
37
139
trm∣Q6IB74
ORM2 protein
1.48
0.00
1.23
38
22
spt∣Q8WZ75
Roundabout homolog 4
0.34
0.00
1.23
39
29
spt∣P02791
Hemopexin
1.73
0.01
1.33
40
33
trm∣Q6LBL5
GM2 activator protein
1.45
0.00
1.06
41
19
pdb∣1HP7_A
A Chain A, uncleaved alpha-1-antitrypsin
1.76
0.00
1.18
42
23
spt∣P55290
Cadherin-13
0.76
0.00
1.13
43
21
trm∣Q8IZY7
Poly-Ig receptor
0.63
0.00
1.36
44
25
spt∣P05154
Plasma serine protease inhibitor
0.76
0.00
1.12
45
10
trm∣Q96CZ9
Cadherin 11, type 2, isoform 1 preproprotein
0.44
0.00
1.50
46
16
gb∣AAR84237.2
Truncated epidermal growth factor
0.48
0.00
1.25
47
35
spt∣P24855
Deoxyribonuclease I
0.50
0.00
1.12
48
17
trm∣Q7Z645
Collagen, type VI, alpha 1
0.51
0.00
1.17
49
15
dbj∣BAA19556.1
Immunoglobulin light chain V-J region
1.69
0.01
1.38
50
33
emb∣CAA23842.1
Unnamed protein product
1.43
0.00
1.05
51
32
spt∣P08571
CD14
2.36
0.00
1.62
52
26
trm∣Q6GMX2
Hypothetical protein
0.59
0.01
1.24
53
111
emb∣CAA29873.2
Alpha-1-acid glycoprotein 2
2.25
0.00
1.18
54
7
trm∣Q8WY99
Cathepsin C
1.58
0.00
1.17
55
11
spt∣Q92820
Gamma-glutamyl hydrolase
0.54
0.01
1.33
56
13
spt∣P15586
N-acetylglucosamine-6-sulfatase
1.30
0.02
1.23
57
10
gb∣AAQ88523.1
AQGV3103
0.79
0.05
1.26
58
8
trm∣Q8N2F4
Hypothetical protein PSEC0200
0.71
0.01
1.29
59
20
trm∣Q6MZU6
Hypothetical protein DKFZp686C15213
0.39
0.00
1.28
60
27
trm∣Q6LDS3
APS protein
1.34
0.00
1.08
61
9
pdb∣1L9X_D
Structure Of Gamma-Glutamyl Hydrolase
0.69
0.00
1.12
62
15
spt∣P07339
Cathepsin D
1.38
0.01
1.26
63
10
spt∣P51884
Lumican
0.78
0.01
1.20
64
130
dbj∣BAC85483.1
Unnamed protein product
0.73
0.00
1.14
65
9
pdb∣1ATH_B
B Chain B, Antithrombin Iii
1.29
0.00
1.17
66
15
rf∣NP_001822.2
Clusterin isoform 1
0.63
0.00
1.25
67
23
trm∣Q5VW91
Decay accelerating factor for complement
1.26
0.00
1.09
68
7
spt∣P54802
Alpha-N-acetylglucosaminidase
0.50
0.00
1.25
69
12
spt∣Q16270
IGFBP-7
0.79
0.00
1.14
70
8
trm∣Q5VZE3
Golgi phosphoprotein 2
0.38
0.02
1.46
71
10
spt∣P05543
Thyroxine-binding globulin
1.27
0.00
1.11
72
9
spt∣P02774
Vitamin D-binding protein
2.44
0.00
1.15
73
63
rf∣NP_000573.1
Secreted phosphoprotein 1
0.58
0.00
1.24
74
56
spt∣P02671
Fibrinogen alpha/alpha-E chain
0.67
0.00
1.11
75
10
trm∣Q9UBG3
Tumor-related protein
0.32
0.00
1.67
76
15
trm∣O00391
Quiescin Q6
0.77
0.03
1.25
77
36
trm∣Q5VY30
Retinol binding protein 4, plasma
1.38
0.00
1.04
78
8
rf∣NP_004675.2
SPARC-like 1
0.55
0.03
1.70
79
9
spt∣Q92692
Herpesvirus entry mediator B
0.50
0.00
1.31
80
39
trm∣Q96FE7
HGFL protein
0.73
0.00
1.15
81
6
spt∣P43652
Afamin
4.67
0.00
1.33
82
24
pdb∣1QDD_A
Lithostathine
1.73
0.00
1.20
83
24
spt∣P10153
Nonsecretory ribonuclease
0.83
0.01
1.13
84
8
spt∣P16278
Beta-galactosidase
1.50
0.00
1.20
85
7
trm∣Q5VYK1
Collagen, type XII, alpha 1
0.75
0.00
1.15
86
9
emb∣CAA37914.1
Precursor (AA-19 to 692)
1.91
0.01
1.62
87
15
trm∣Q7Z5L0
Unnamed secretory protein
0.56
0.00
1.31
88
11
spt∣P08236
Beta-glucuronidase
1.33
0.00
1.12
89
13
cra∣hCP51001.2
superoxide dismutase 3
0.45
0.00
1.38
90
17
pir∣S13195
Ganglioside M2 activator protein
1.33
0.00
1.18
91
12
cra∣hCP1858145
Protein C receptor, endothelial
1.42
0.00
1.22
92
12
trm∣Q6IAT8
B2M protein
1.48
0.00
1.08
93
7
trm∣Q9Y5X6
Glutamate carboxypeptidase
1.47
0.00
1.04
94
13
spt∣P06702
Calgranulin B
0.41
0.00
1.36
95
11
emb∣CAB90482.1
Human type XVIII collagen
0.56
0.01
1.48
96
7
trm∣O00533
Neural cell adhesion molecule
0.73
0.02
1.29
97
72
spt∣P04745
Salivary alpha-amylase
1.25
0.00
1.12
98
40
spt∣O75594
Peptidoglycan recognition protein
0.56
0.00
1.08
99
127
emb∣CAA40946.1
Immunoglobulin lambda light chain
1.71
0.01
1.47
100
15
trm∣Q9UJ36
Transmembrane glycoprotein
0.74
0.01
1.25
101
4
gb∣AAH17802.1
SPRR3 protein
0.14
0.00
1.25
102
9
spt∣Q01469
Fatty acid-binding protein
0.29
0.00
1.34
103
4
trm∣Q6FGL5
LCN2 protein
1.36
0.01
1.33
104
10
trm∣Q9UMV3
MBL-associated serine protease 2
0.29
0.00
1.34
105
10
gb∣AAH30653.1
Cadherin 13, preproprotein
2.91
0.00
1.76
106
6
spt∣Q9H8L6
Multimerin 2
0.69
0.02
1.34
107
33
trm∣Q9UD19
Intron-containing kallikrein
0.73
0.02
1.30
108
4
spt∣P07195
L-lactate dehydrogenase B chain
0.75
0.00
1.16
109
9
spt∣P08185
Corticosteroid-binding globulin
3.22
0.00
1.79
110
14
trm∣Q5UGI3
Ubiquitin C splice variant
1.37
0.01
1.24
111
6
pdb∣1O1P_D
D Chain D, Deoxy Hemoglobin
0.55
0.00
1.32
112
7
spt∣P80723
Brain acid soluble protein 1
2.24
0.00
1.46
113
7
spt∣P19320
Vascular cell adhesion protein 1
1.25
0.03
1.23
114
6
spt∣P27797
Calreticulin
1.39
0.00
1.13
115
10
spt∣Q01459
Di-N-acetylchitobiase
1.43
0.01
1.26
116
6
trm∣Q6PN97
Alpha 2 macroglobulin
3.04
0.00
1.84
117
5
gb∣AAV40827.1
superoxide dismutase 3
0.48
0.00
1.15
118
3
spt∣P08473
Neprilysin
2.26
0.00
1.51
119
61
trm∣Q9Y5Y7
LYVE-1
1.63
0.00
1.03
120
2
spt∣P26038
Moesin
1.56
0.00
1.18
121
9
trm∣Q6PIJ0
FCGR3A protein
0.77
0.00
1.06
122
7
spt∣P14209
T cell surface glycoprotein E2
2.02
0.02
1.61
123
6
spt∣P02765
Alpha-2-HS-glycoprotein
1.69
0.00
1.20
124
215
pir∣A23746
Ig kappa chain V-III
1.31
0.00
1.14
125
3
trm∣Q9NT71
Hypothetical protein DKFZp761A051
0.74
0.03
1.31
126
6
trm∣Q9Y4W4
Type XV collagen
0.52
0.00
1.25
127
5
cra∣hCP42501.1
Complement component 1
0.74
0.00
1.20
128
11
spt∣P09564
T-cell antigen CD7
0.67
0.01
1.26
129
6
dbj∣BAA86053.1
Carboxypeptidase E
0.79
0.02
1.20
130
6
spt∣P15151
Poliovirus receptor
1.46
0.01
1.51
131
13
trm∣Q8IUP2
Protocadherin 1, isoform 1
0.49
0.02
1.75
132
3
trm∣Q8NBK0
Hypothetical protein
1.31
0.01
1.19
133
5
spt∣P22891
Vitamin K-dependent protein Z
0.76
0.01
1.21
134
6
trm∣Q9UNF4
Hyaluronic acid receptor
1.50
0.01
1.29
135
11
trm∣Q9HCU0
Tumor endothelial marker 1
0.46
0.00
1.36
136
8
spt∣P35527
Keratin, type I cytoskeletal 9
0.55
0.00
1.29
137
3
spt∣P55285
Cadherin-6
0.41
0.00
1.39
138
3
trm∣Q9BYH7
Scavenger receptor with C-type lectin type I
1.39
0.01
1.23
139
2
trm∣Q13942
Calmodulin
2.64
0.00
1.39
140
8
spt∣P04004
Vitronectin
1.87
0.00
1.42
141
11
trm∣Q86Z23
Hypothetical protein
2.11
0.01
1.49
142
140
trm∣Q9NWE3
Hypothetical protein FLJ10084
1.55
0.00
1.04
143
99
trm∣Q9NWE3
Hypothetical protein FLJ10084
1.53
0.00
1.06
144
11
spt∣P05109
Calgranulin A
0.42
0.00
1.34
145
58
trm∣Q9NWE3
Hypothetical protein FLJ10084
1.50
0.00
1.08
146
3
trm∣Q9BYH7
Scavenger receptor with C-type lectin type I
1.65
0.00
1.24
147
4
gb∣AAA52018.1
Chromogranin A
0.52
0.00
1.26
148
4
emb∣CAI20248.1
PPGB
1.51
0.00
1.24
149
92
pir∣S12443
Ig lambda chain (Ke+O−)−human
1.35
0.03
1.24
150
7
trm∣Q5TEQ5
OTTHUMP00000044363
0.29
0.00
1.58
151
18
trm∣Q6UX86
GPPS559
2.08
0.01
1.60
152
2
trm∣Q8TCZ2
MIC2L1
0.80
0.01
1.16
153
5
spt∣P61970
Nuclear transport factor 2
0.78
0.01
1.18
154
284
pdb∣1T04_C
Anti-ifn-gamma fab in C2 space group
1.45
0.02
1.28
155
8
spt∣P00790
Pepsin A
0.80
0.00
1.15
156
2
cra∣hCP1778903.1
CD7 antigen
0.48
0.00
1.03
157
5
trm∣O00480
Butyrophilin, subfamily 2
0.68
0.00
1.12
158
5
trm∣O43653
Prostate stem cell A
1.70
0.00
1.25
159
4
trm∣Q9BX83
Hemoglobin alpha 1 globin chain
0.67
0.05
1.47
160
9
spt∣P11684
Uteroglobin
3.16
0.00
1.86
161
7
trm∣Q7LDY7
Alpha-KG-E2
1.47
0.00
1.17
162
3
trm∣Q9BYH7
Scavenger receptor
1.92
0.00
1.25
163
4
trm∣Q6AZK5
KRT13 protein
0.56
0.02
1.58
164
5
spt∣P09619
PDGF-R-beta
0.66
0.01
1.29
165
5
pdb∣5TTR_H
Leu 55 Pro Transthyretin
1.46
0.02
1.33
166
3
rf∣NP_877418.1
Mucin 1, transmembrane
0.49
0.02
1.78
167
2
trm∣Q5SWW9
OTTHUMP00000060590
0.39
0.01
1.76
168
2
rf∣NP_003217.2
Trefoil factor 3
1.63
0.00
1.22
169
3
spt∣Q99574
Neuroserpin
0.63
0.01
1.36
170
21
trm∣Q9Y3U9
Hypothetical protein DKFZp566C243
0.75
0.00
1.06
171
23
gb∣AAB27607.1
Prostaglandin D synthase
1.20
0.00
1.10
172
4
gb∣AAO11857.1
Immunoglobulin
1.38
0.00
1.14
173
2
trm∣Q5VTA6
Cubilin
0.79
0.03
1.23
174
3
trm∣Q5SY67
OTTHUMP00000059857
0.69
0.02
1.33
175
54
spt∣P15814
Immunoglobulin lambda-like polypeptide 1
2.50
0.01
1.37
176
2
spt∣P22352
Plasma glutathione peroxidase
0.70
0.00
1.13
177
7
gb∣AAL68978.1
Mutant beta globin
0.32
0.00
1.77
178
6
spt∣P35908
KCytokeratin 2e
0.50
0.03
1.74
179
284
dbj∣BAB18261.1
Anti-HBs antibody light chain
3.19
0.00
1.53
180
3
rf∣XP_370615.2
Hypothetical protein
0.53
0.03
1.72
181
164
gb∣AAB50880.2
Anitubulin IgG1 kappa VL chain
1.64
0.00
1.30
182
170
dbj∣BAC01692.1
Immunoglobulin kappa light chain
2.49
0.01
1.79
183
2
trm∣Q7RTN9
Type II keratin K6h
0.43
0.01
1.52
184
3
spt∣P21926
Motility-related protein
1.70
0.03
1.45
185
2
spt∣Q13873
Bone morphogenetic protein receptor
0.61
0.01
1.08
186
4
gb∣AAA62175.1
Heat shock protein 27
0.53
0.00
1.38
187
15
spt∣P02787
transferrin
1.86
0.00
1.25
188
2
spt∣P07108
Acyl-CoA-binding protein
2.20
0.02
1.47
189
2
spt∣Q9NZH0
G protein-coupled receptor family C
1.27
0.04
1.25
190
2
trm∣Q96E46
Fructose-1,6-bisphosphatase 1
0.44
0.00
1.01
191
44
gb∣AAB53267.1
Immunoglobulin V-region light chain
1.40
0.03
1.30
192
8
gb∣AAR32503.1
Immunoglobulin heavy chain
0.46
0.00
1.19
193
7
rf∣NP_653247.1
Immunoglobulin J chain
0.37
0.01
1.88
194
29
emb∣CAA12585.1
Ig heavy chain variable region
1.50
0.00
1.13
195
4
gb∣AAD16731.1
Immunoglobulin lambda light chain
1.58
0.00
1.11
196
33
trm∣Q6UXB8
HGSC289 (OTTHUMP00000039678)
1.27
0.04
1.22
aThe numbers of unique peptides and MS/MS spectrum observed by ProteinPilot software were determined only for those peptides with ≥95% confidence. bAccession numbers represent entries in the Human CDS database (human KBMS 5.0, 2005-03-02; a total of 187,748 entries provided by Applied Biosystems). c–eThe iTRAQ ratio, value, and EF value in microalbumiuric versus normoalbuminuric urine, respectively.
