201295.fig.001
Figure 1: Schematic representations of the differentiation protocol from ES cells into insulin-producing cells. Undifferentiated feeder-free ES-D3 cells were cultured in collagen-I-coated plates and incubated in differentiation DMEM/F-12 medium supplemented with 2 mM L-glutamine, 100 μM nonessential amino acids, 10 ng/mL activin A, 10 mM nicotinamide, and 1 μg/mL laminin with 10% FBS overnight. ES-D3 cells were next exposed to DMEM/F-12 medium supplemented with 2 mM L-glutamine, 100 μM nonessential amino acids, 10 ng/mL activin A, 10 mM nicotinamide, 25 μg/mL insulin, and 1 μg/mL laminin with 2% FBS for 6 days. Differentiated ES-D3 cells formation of islet-like clusters at 7 days. Bar = 50 μm.