201295.fig.004a
(a)
201295.fig.004b
(b)
Figure 4: Intracellular insulin content. Insulin secretion was measured by ELISA and normalized to total cellular protein. (a) Insulin-producing cells were incubated with KRBH buffer containing glucose and 50 μM tolbutamide for 1.5 hours. Bars represent means ± SD from three independent experiments ( ). * significantly different from glucose. (b) Insulin-producing cells were incubated with 3 μM LY294002 or not (control) for 2 hours. Bars represent means ± SD from three independent experiments ( ). * significantly different from control.