Efficient Differentiation of Mouse Embryonic Stem Cells into Insulin-Producing Cells
Figure 4
Intracellular insulin content. Insulin secretion was measured by ELISA and normalized to total cellular protein. (a) Insulin-producing cells were incubated with KRBH buffer containing glucose and 50 μM tolbutamide for 1.5 hours. Bars represent means ± SD from three independent experiments (). * significantly different from glucose. (b) Insulin-producing cells were incubated with 3 μM LY294002 or not (control) for 2 hours. Bars represent means ± SD from three independent experiments (). * significantly different from control.
