TXNIP Links Innate Host Defense Mechanisms to Oxidative Stress and Inflammation in Retinal Muller Glia under Chronic Hyperglycemia: Implications for Diabetic Retinopathy
Figure 7
HG-induced TXNIP expression correlates with proapoptotic caspase-3 expression in rMC1. (a) IHC of TXNIP. Txnip staining is enhanced in rMC1 under HG exposure for 5 days, which correlates with increased (b) proapoptotic caspase 3 staining in similar duration of HG using a caspase-3 FLICA staining kit. A representative of is shown here. (c–f) HG induces TXNIP and pro-inflammatory gene expression in rMC1. Total RNA was isolated with TRIZOL and mRNA levels for (c) TXNIP, (d) iNOS, (e) Cox-2, and (f) VEGF-A were measured by qRT-PCR at various time periods of HG exposure (). values were compared against respective controls at time 0.
