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Figure 2: Time course of the effect of metformin on FGF21 expression. FGF21 mRNA expression in primary rat hepatocytes incubated with 1000 μM metformin for 0, 4, 6, 10, and 24 hrs. Data are means ± SEM; versus non-treated hepatocytes; ns, non-significant, analyzed by paired Student’s t-test, .