Dysregulation of Dicer1 in Beta Cells Impairs Islet Architecture and Glucose Metabolism

Figure 1

Dicer1 conditional knockout in beta cells, results in impaired glucose tolerance and reduction in beta-cell mass. A diagram of mouse genetics for generation of RIP-Cre; Dicer1^LoxP/LoxP mutants that additionally harbor Rosa-EYFP reporter (a). Blood glucose levels of RIP-Cre; Dicer1^LoxP/LoxP mutants and RIP-Cre; Dicer1^LoxP/+ controls, after overnight fasting or when randomly fed (b), and during standard glucose tolerance test (GTT) at multiple time-points after intra-peritoneal injection of a glucose bolus (c). Assessment of the percentage of insulin-positive sectional area relative to the total pancreas area in serial sections of mutant and control pancreata (d)). Calculated beta-cell mass, corrected to pancreas weight (e). *𝑃<0.05.

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