Modulation of Apoptosis Pathways by Oxidative Stress and Autophagy in β Cells
Figure 1
Stress-induced modulation of genes in apoptosis pathway. Human islets (2000 IEQ) cultured in Miami medium were exposed to multiple stresses including peroxynitrite (PN; 200 μM), H2O2 (200 μM), or a mixture of cytokines (Cyt; 2 ng/mL of IL-1β, 10 ng/mL of TNF-α, and 10 ng/mL of IFN-γ) for 24 h or cultured under hypoxic conditions (1% oxygen) for 8 h. RNA was isolated from treated islets for the RT-PCR analysis of BFAR, CARD8, CIDE-A, and BNIP3 using Taqman probes. Results are M ± SE of experiments with four independent batches of human islets. *; ** when compared to untreated control (Con).
