Combined Transfection of the Three Transcriptional Factors, PDX-1, NeuroD1, and MafA, Causes Differentiation of Bone Marrow Mesenchymal Stem Cells into Insulin-Producing Cells
Figure 5
Expression of insulin protein in the infected cells. After culturing them for 21 days, all the infected cells were incubated with anti-mouse insulin antibody. Nuclei were stained blue with Hoechst dye (a). Most of the transgenic cells were stained positively for insulin (b). In contrast, the mMSCs infected with Ad-GFP or null were negative for insulin.
