Pentoxifylline Attenuates Methionine- and Choline-Deficient-Diet-Induced Steatohepatitis by Suppressing TNF-α Expression and Endoplasmic Reticulum Stress

Figure 1

Viability of Hep3B cells after treatment with TNF-α and pentoxifylline. Hep3B cells exposed to 10, 20, 50, 100, and 200 ng/mL TNF-α for 24 hours showed a significant decrease in cell viability, assessed by MTT. Pretreatment with 1 mM pentoxifylline for 2 and 4 h prevented TNF-α-induced Hep3B toxicity. The bars represent percent cytotoxicity versus untreated controls. The experiments were performed four times under identical conditions. Results are shown as mean ± SEM. ***P < 0.001 versus untreated control cells; ^†††𝑃 < 0.001 versus 1 mM pentoxifylline for 2 h; ^‡‡‡𝑃 < 0.001 versus 1 mM pentoxifylline for 4 h.