Regulation of LYRM1 Gene Expression by Free Fatty Acids, Adipokines, and Rosiglitazone in 3T3-L1 Adipocytes

Figure 1

The expression of the LYRM1 mRNA during the conversion of 3T3-L1 cells to adipocytes. 3T3-L1 cells were induced to differentiate, as described in “Materials and Methods’’ section. Total RNA was harvested from the 3T3-L1 cells on alternate days before (day −2, day 0) and after (day 2, day 4, day 6, day 8, day 10, and day 12) the switch from growth medium to differentiation medium. LYRM1 mRNA levels were analyzed using quantitative real-time RT-PCR and normalized to β-actin levels. The results are presented as the means ± SE of six experiments.