Inhibition of Protein Tyrosine Phosphatase Improves Angiogenesis via Enhancing Ang-1/Tie-2 Signaling in Diabetes
Figure 2
Immunoprecipitation and Western blot analysis showing that SHP-1 associates with Tie-2 receptor and high glucose alters the SHP-1/Tie-2 association in MHMEC. (a) SHP-1 binds to the Tie-2 receptor under normal glucose (NG) conditions; exposure of MHMEC to high glucose (HG) leads to a significant increase in the SHP-1/Tie-2 association ( cell lines, *). (b) Exposure of MHMEC to high glucose (HG) conditions results in a significant reduction of Tie-2 phosphorylation ( cell lines, *). (c) Under normal glucose (NG) conditions, stimulation of MHMEC with Ang-1 (250 ng/mL) for 15 minutes leads to a dissociation of SHP-1 from Tie-2. Under HG conditions, Ang-1 failed to induce SHP-1/Tie-2 dissociation. These results represent three different cell lines and experiments.
