Inhibition of Protein Tyrosine Phosphatase Improves Angiogenesis via Enhancing Ang-1/Tie-2 Signaling in Diabetes

Figure 4

Inhibition of PTP enhances Ang-1-induced Akt and eNOS phosphorylation under HG conditions. (a) Western blot showing that pretreatment of MHMEC with the PTP inhibitor orthovanadate (OV, 5 μM) for 30 minutes leads to an increase in Akt phosphorylation in response to Ang-1 (250 ng/mL) stimulation under high glucose conditions (𝑛=3, *𝑃<0.05). (b) Western blot showing that pretreatment of MHMEC with the PTP inhibitor OV for 30 minutes results in a significant increase in Ang-1-induced eNOS phosphorylation under high glucose conditions (𝑛=3, *𝑃<0.05).

(a)

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