Effect of propionate treatment (0.3 g/kg, i.p. for 7 days) on Akt knockout-induced change in pan and phosphorylated Akt and PTEN. (a) Pan Akt; (b) pan PTEN; (c) phosphorylated Akt (pAkt); (d) phosphorylated PTEN (pPTEN); (e) pAkt-to-Akt ratio; (f) pPTEN-to-PTEN ratio. Insets: representative gel blots depicting expression and phosphorylation of these proteins using specific antibodies. β-Actin was used as the loading control. Mean ± SEM, mice per group, and *P < 0.05 versus WT group.
