Haplotypes in the CRP Gene Associated with Increased BMI and Levels of CRP in Subjects with Type 2 Diabetes or Obesity from Southwestern Mexico

Table 1

PCR primers, products, and restriction enzymes for the genotyping of CRP polymorphisms.

SNP	Forward and reverse primers	PCR product (bp)	Restriction enzyme	Fragments; (bp)			Reference
rs1130864	5′-AGCTCGTTAACTATGCTGGGGCA-3′; 5′-CTTCTCAGCTCTTGCCTTATGAGT-3′	181	𝐻𝑝𝑦𝐶𝐻4  III	CC; 181	CT; 181, 156, 25	TT; 156, 25	[9]
rs1205	5′-GGAGTGAGACATCTTCTTG-3′; 5′-CTTATAGACCTGGGCAGT-3′	227	𝐻𝑝𝑦𝐶𝐻4  III	GG; 130 y 97	GA; 227, 130, 97	AA; 227	[17]
rs2794521	5′-GCCGTCATTTAGTGCCAAC-3′; 5′-ATGCTCCTCCCAGAGCCATGG-3′	376	Bstul	AA; 376	AG; 376, 199, 177	GG; 199, 177	[18]
rs3093062	5′-TTTGGGCTAAGTAGGTGTTG-3′; 5′-AGGGCTCCACTTTGGCTATC-3′	116	Apal I	GG; 38, 78	GA; 116	AA; 38, 78, 116	[8]