Research Article

Ampicillin-Improved Glucose Tolerance in Diet-Induced Obese C57BL/6NTac Mice Is Age Dependent

Figure 7

Flow cytometric analyses of lymphocytes isolated from the spleen, mesenteric lymph nodes (MLN), and Peyer’s patches (PP). ((a)–(c)) Percentages of CD11b positive dendritic cells (CD11c+). ((d)–(f)) Percentages of tolerogenic CD103 positive dendritic cells. ((g)–(i)) Percentages of FoxP3 positive regulatory T cells (CD4+). High-fat diet (HFD) induced C57BL/6 mice treated with Ampicillin from birth and until five weeks of age (1A; Ampicillin 5w+ 16w−/DIO+), or from birth and until five weeks of age followed by Ampicillin treatment from 12 to 16 weeks of age (1B; Ampicillin 5w+ 16w+/DIO+), or only from 12 to 16 weeks of age (2A; Ampicillin 5w− 16w+/DIO+) are illustrated together with untreated HFD induced mice (2B; Ampicillin 5w− 16w−/DIO+) and untreated control mice (3; Ampicillin 5w− 16w−/DIO−). Error bars represent the SEM. *( ), **( ).
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