Ampicillin-Improved Glucose Tolerance in Diet-Induced Obese C57BL/6NTac Mice Is Age Dependent

Table 1

Serum cytokines in diet-induced obese C57BL/6 mice treated or not treated with Ampicillin from birth to five weeks of age, from 12 to 16 weeks of age, or both.

Group	Ampicillin		DIO	𝑁	IL-1a			IL-2			IL-4
	Early	Late			Median	Minimum	Maximum	Median	Minimum	Maximum	Median	Minimum	Maximum
1A	+	−	+	10	80.845	28.35	591.01	31.44	2.91	66.11	2.17	0.35	31.05
1B	+	+	+	10	61.96	7.85	591.01	32.61	4.4	53.17	3.26	0.35	22.25
2A	−	+	+	6	100.32	28.76	664.78	32.27	4.4	124.6	0.35	0.35	5.49
2B	−	−	+	4	112.92	45.67	2222.22	4.4	4.4	43.81	1.625	0.35	3.26
3	−	−	−	8	62.395	45.6	389.82	36.56	5.34	65.84	3.82	0.35	28.73
Group	Ampicillin		DIO	𝑁	IL-5			IL-6			IL-10
	Early	Late			Median	Minimum	Maximum	Median	Minimum	Maximum	Median	Minimum	Maximum
1A	+	−	+	10	214.27	2	57.4	37.55	1.1	136.14	155.045	48.06	874.79
1B	+	+	+	10	87.08	32.08	60.14	34.3	1.1	374.97	246.91	70.73	1244.12
2A	−	+	+	6	269.025	55.23	36.38	9.2	1.1	80.87	352.825	146.38	729.41
2B	−	−	+	4	140.725	13.46	4.3	6.7^a	1.1	12.3	205.345	114.05	1883.25
3	−	−	−	8	164.635	2	4.3	34.05^a	1.1	51.72	403.235	130.14	642.39
Group	Ampicillin		DIO	𝑁	IL-12p70			IL-17			IL-18
	Early	Late			Median	Minimum	Maximum	Median	Minimum	Maximum	Median	Minimum	Maximum
1A	+	−	+	10	4.3	4.3	57.4	11.24	1.2	25.05	520.83	58.45	1386.39
1B	+	+	+	10	4.3	4.3	60.14	9.21	0.73	28.9	58.45	58.45	1902.05
2A	−	+	+	6	4.3	4.3	36.38	2.425	1.2	20.53	58.45	58.45	1939.3
2B	−	−	+	4	4.3	4.3	4.3	3.485	2.22	4.97	58.45	58.45	4876.61
3	−	−	−	8	4.3	4.3	4.3	8.25	1.2	18.87	58.45	58.45	1939.3
Group	Ampicillin		DIO	𝑁	INF𝛾			TNF𝛼			GM-CSF
	Early	Late			Median	Minimum	Maximum	Median	Minimum	Maximum	Median	Minimum	Maximum
1A	+	−	+	10	44.675	5.56	252.73	1.05	1.05	1.05	5.45	5.45	49.69
1B	+	+	+	10	35.04	3.03	444.26	1.05	1.05	1.05	5.45	5.45	24.7
2A	−	+	+	6	35.085	17.95	436.95	1.05	1.05	1.05	5.45	3.74	24.7
2B	−	−	+	4	36.99	24.64	1421.42	1.05	1.05	1.05	5.45	5.45	6.29
3	−	−	−	8	35.915	21.2	333.55	1.05	1.05	1.05	5.45	3.74	24.7

^a𝑃=0.039.