Ampicillin-Improved Glucose Tolerance in Diet-Induced Obese C57BL/6NTac Mice Is Age Dependent

Table 2

Genes tested by qPCR in ileum tissue of in C57BL/6 mice treated or not treated with Ampicillin from birth to five weeks of age, from 12 to 16 weeks of age, or both.

Gene symbol	Gene	Amplification efficiency (%)		Sequence 5′-3′	Accession number
Actb	Beta-actin	99	F	CCCTAAGGCCAACCGTGAAA	NM_007393.3
			R	CAGCCTGGATGGCTACGTAC
Alpi	Alkaline phosphatase,	93	F	TCCTAAAGGGGCAGTTGGAA	NM_001081082.1
	Intestinal		R	ACCTGTCTGTCCACGTTGTA
B2m	Beta-2 microglobulin	100	F	CTGGTGCTTGTCTCACTGAC	NM_009735.3
			R	GGTGGGTGGCGTGAGTATA
Gusb	Glucuronidase, beta	101	F	AGTATGGAGCAGACGCAATCC	NM_010368.1
			R	ACAGCCTTCTGGTACTCCTCA
Hp	Haptoglobin	96	F	TATCGCTGCCGACAGTTCTAC	NM_017370.2
			R	CTCTCCAGCGACTGTGTTCA
Hprt1	Hypoxanthine	97	F	CAGTACAGCCCCAAAATGGTTA	NM_013556.2
	Phosphoribosyltransferase		R	AGTCTGGCCTGTATCCAACA
Il18	Interleukin 18	98	F	CAAAGAAAGCCGCCTCAAAC	NM_008360.1
			R	GACGCAAGAGTCTTCTGACA
Il1a	Interleukin 1 alpha	103	F	AGATGGCCAAAGTTCCTGAC	NM_010554.4
			R	AGAGATGGTCAATGGCAGAAC
Lct	Lactase	92	F	TGTCCTAGCCTACAACCTCAAC	NM_001081078
			R	AGCGGTCTGTAATGGAAGCA
Muc2	Mucin 2	93	F	TATGCCAGGCCAGGAGTTTA	NM_023566.2
			R	GCAAGGCAGGTCTTTACACA
Nfkbia	Nuclear factor of kappa, alpha	96	F	GAGCGAGGATGAGGAGAGCTA	NM_010907.2
			R	GGCCTCCAAACACACAGTCA
Rpl13a	Ribosomal protein L13A	103	F	AGGTTACGGAAACAGGCAGAA	NM_009438.
			R	CAGGAGTCCGTTGGTCTTGA
Saa	Serum amyloid A	102	F	GAGTCTGGGCTGCTGAGAAA	NM_011314.2
			R	ATGGTGTCCTCGTGTCCTCT
Tbp	TATA box binding protein	100	F	ACCAGAACAACAGCCTTCCA	NM_013684.3
			R	AAAGATGGGAATTCCAGGAGTCA
Tgfb1	Transforming growth factor, beta 1	98	F	GCTGCGCTTGCAGAGATTAA	NM_011577.1
			R	GTAACGCCAGGAATTGTTGCTA
Tlr4	Toll-like receptor 4	98	F	GTTCTTCTCCTGCCTGACAC	NM_021297.2
			R	GCTGAGTTTCTGATCCATGCA
Tnf	Tumor necrosis factor	95	F	CAAATGGCCTCCCTCTCATCA	NM_013693.2
			R	TGGGCTACAGGCTTGTCAC
Tnfsf15	Tumor necrosis factor	97	F	GCAAGCCGAGAGCACAC	NM_177371.3
	Superfamily, member 15		R	CCATCCCTAGGTCATGTTCCC