IL-10 Induction from Implants Delivering Pancreatic Islets and Hyaluronan
Figure 5
Local release of fluid HA from BIs generates IL-10 producing T cells in vivo. (a) Diagram of the experiment. BIs incorporating RIPmOVA islets and alginate spheres loaded with HA or saline (controls) were implanted in RIPmOVA /Rag2−/− mice, followed by injection of OVA-specific DO11.10 T cells on day 1 and 100 g of OVA peptide in incomplete Freund’s adjuvant (IFA) on day 2. On day 14, splenocytes were harvested and assayed for IL-10 production in response to antigenic stimulation in vitro. (b) IL-10 production by splenocytes from recipient animals in response to stimulation with anti-CD3/CD28 antibodies. (c) IL-10 production in response to OVA peptide (black bars) or no peptide (white bars). In (b) and (c), cells were stimulated for 96 hours, and culture supernates in contact with the stimulated cells for 72 hours were assayed by ELISA. Data are representative of 2 experiments totaling 5 mice in each group.
