Impact of High Glucose and Proteasome Inhibitor MG132 on Histone H2A and H2B Ubiquitination in Rat Glomerular Mesangial Cells

Figure 4

The expression of histone H2A and H2B ubiquitination in GMCs induced by 30 mmol/L glucose at various times determined using Western blot. (a) Compared to the NC group, uH2A expression increased after stimulation with 30 mmol/L glucose after 24 hr, but the uH2B expression decreased over time. (b) The gray graph shows the relative statistical values for uH2A protein expression at various time points for each group. The expression of uH2A was increased at 24 h sharp. ^∗𝑃<0.01 versus NC group, ^#𝑃<0.05 versus 12 h, and ^※𝑃<0.05 versus 24 h. (c) The gray graph shows the relative statistical values for uH2B protein expression at various time points for each group. And the change of uH2B expression was a time dependent reduction. ^∗𝑃<0.01 versus NC group, ^#𝑃<0.05 versus 12 h, and ^※𝑃<0.05 versus 24 h.

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