Application of IgG-Derived Natural Treg Epitopes (IgG Tregitopes) to Antigen-Specific Tolerance Induction in a Murine Model of Type 1 Diabetes

Figure 8

ELISpot results from a recently diagnosed T1D patient. Dark bars represent cells preincubated with a pool of GAD65-derived epitopes, and light bars represent cells precultured with the same pool plus Tregitope peptides. IFNγ ELISpot responses to GAD65 peptides incubated with PBMCs from a recently diagnosed subject (<6 months) were either low or did not meet criteria for a positive response. Tregitopes were able to suppress responses to the whole GAD65 protein and to the GAD65 peptides, individually or pooled.