Research Article

Variations in Rodent Models of Type 1 Diabetes: Islet Morphology

Figure 8

Islet size and cell composition in STZ-treated rats. (a) An islet from nondiabetic DA rat was large with a normal cellular morphology. Scale bar = 100 μm for all images. (b) A representative islet from a normoglycemic STZ-treated rat was smaller, but still maintained the β-cell core (green) with the α- (red) and δ-cells (blue) rim. (c) Typical islet from hyperglycemic rat was smaller than the other 2 groups with few β-cells. (d) Islet diameter was plotted, and each group was more statistically different than the others. (e) The number of cells per islet area was less in the 2 STZ-treated groups (DN and DH) compared to controls. (f) Cell composition was plotted showing a loss of β-cells with hyperglycemia. Cell composition from each of the 3 groups was different for each cell type except for the δ-cells between the DN and DH groups (NS = not significant). of islets = 96 from 3 controls, 73 from 3 DN rats, and 74 in the DH group (3 rats).
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