The Visualization of Biofilms in Chronic Diabetic Foot Wounds Using Routine Diagnostic Microscopy Methods
Figure 2
Images acquired from Sample 1. (a) and (b) are replicate images from Gram-stained sections; (c) and (d) (replicates) have been visualized using a combination of FISH (red), to indicate eubacteria, ConA (green) to indicate biofilm matrix, and other ConA-reactive material, and with Hoechst 33252 (blue) for the detection of nucleic acids. (e) and (f) show replicate ESEM images. Presumptive bacterial microcolonies and biofilm matrix have been indicated by arrows.
