Adipocytes from New Zealand Obese Mice Exhibit Aberrant Proinflammatory Reactivity to the Stress Signal Heat Shock Protein 60
Figure 2
Characterization of the interaction of different Hsp60 species with preadipocytes from C57BL/6J, NOD, and NZO mice. C57BL/6J (light grey bars), NOD (dark grey bars), and NZO mouse-derived cells (black bars) were preincubated in the absence (Medium) or presence of 1 μM unlabeled eukaryotic or prokaryotic Hsp60 species (human, mouse, rat, hamster, E. coli, and M. bovis), followed by addition of 100 nM human Hsp60-DyLight649 (Hsp60*). Hsp60* binding was quantified by FACS analysis. Data represent residual Hsp60* binding as percent of the binding of Hsp60* in the absence of Hsp60 or OVA (set as 100%, white bar). The results are shown as means + SEM of ≥ three independent experiments. *; ** compared to Hsp60* binding in the absence of Hsp60 and OVA.
