Low Molecular Weight Fucoidan Alleviates Cardiac Dysfunction in Diabetic Goto-Kakizaki Rats by Reducing Oxidative Stress and Cardiomyocyte Apoptosis

Figure 2

LMWF attenuated pathological changes in the hearts of diabetic GK rats. Representative micrographs of myocardial tissue sections stained with Hematoxylin and Eosin (a) and Sirius Red (b) for each group (, scale bar = 100 μm). I represents control group, II represents DM group, and III and IV represent diabetic rats administered with 50 and 100 mg/kg/day LMWF, respectively. The black lined-box (in (b)) shows a high-magnification micrograph of Sirius Red-stained sections. Myocardial fibrosis was stained in red. (c) Fibrosis area was measured and statistical analysis was performed in different groups. (d) The level of fibronectin mRNA expression in heart tissues of each group was determined by real-time PCR, and the statistical analysis of fibronectin mRNA normalized to the housekeeping gene GAPDH was shown (). , versus control group; , versus DM group.

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