Hyperglycemia Inhibits Complement-Mediated Immunological Control of S. aureus in a Rat Model of Peritonitis

Figure 4

Opsonization of S. aureus recovered from the peritoneum. (a) Washed bacteria were stripped of bound C4-fragments and assayed by ELISA. (b) Washed bacteria were analyzed by flow cytometry after incubation with anti-C3 antibody. Area under the curve was measured for each sample (log scale). (c) Washed bacteria were stripped of bound C3-fragments and assayed by ELISA. Graphed data shows means of results for peritoneal samples from the animals in each treatment group. Error bars denote standard error of the means. (d) Total C3-fragment Western blot of stripped bound C3-fragments from representative samples revealing predominantly iC3b and C3d.

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