Research Article
[Retracted] Hyperglycemia Induces Toll-Like Receptor-2 and -4 Expression and Activity in Human Microvascular Retinal Endothelial Cells: Implications for Diabetic Retinopathy
Figure 9
Antioxidant treatment attenuates HG-mediated ROS and TLR-2 and TLR-4 activation: HMVRECs treated with apocynin (60 M) and N-acetyl cysteine (10 mM) and subsequently exposed to high glucose. (a) ROS measurement by H2DCFDA staining. versus control and versus 25 mM; (b) TLR-2 and TLR-4 protein levels measured using western blot. versus control and versus 25 mM; (c) TLR-2 and TLR-4 mRNA expression measured by PCR. versus control and versus 25 mM. (d) NF-B p65 measurement by ELISA, versus control and versus 25 mM.
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