Beneficial Effects of Adiponectin on Periodontal Ligament Cells under Normal and Regenerative Conditions
Figure 2
Effects of EMD and/or adiponectin (3 μg/mL) on wound closure over 4 d (a). Untreated cells were used as control. Mean ± SEM (); *significantly () different from cells treated with EMD, either alone or with adiponectin; #significantly () different from all other groups. Effects of EMD and/or adiponectin (1 μg/mL) on the mRNA expression of Ki67 at 1 d and 3 d (b). Untreated cells were used as control. Mean ± SEM (); *significant () difference between groups. Effects of EMD on SMAD1/5/8 nuclear translocation in the presence and absence of adiponectin (1 μg/mL) at 60 min, as analyzed by immunofluorescence (c–f). Untreated cells served as control. Experiments were performed in triplicate and repeated twice. Images from one representative donor are shown.
