Polymorphisms of GLP-1 Receptor Gene and Response to GLP-1 Analogue in Patients with Poorly Controlled Type 2 Diabetes

Figure 1

Experimental confirmation of the dinucleotide repeat polymorphism. A 2 bp deletion at chromosome 6: 39047037-39047052 was detected. (a) PCR direct sequencing chromatography trace. (b), (c) The PCR products were cloned, and at least 10 single colonies were analyzed by DNA sequencing. One plasmid contained the wild type sequence ((b), 8GA), whereas the other plasmids contained a deletion of GA ((c), 7GA). The underlined sequences indicate the one unit of the GA sequences.

(a)

(b)

(c)