NADPH Oxidase-Induced NALP3 Inflammasome Activation Is Driven by Thioredoxin-Interacting Protein Which Contributes to Podocyte Injury in Hyperglycemia

Figure 5

Genetic or pharmacologic inhibition of NADPH oxidase alleviates HG-induced podocyte injury. (a) Western blotting gel documents showing the effect of gene deletion on desmin and synaptopodin expression in cultured human podocytes with HG treatment. (b) Summarized data of protein expression of desmin and synaptopodin in podocytes ( = 4–6). (c) Western blot analysis showing the effect of APO and DPI on desmin and synaptopodin expression in podocytes exposed to HG. (d) Summarized data of the abundance of desmin and synaptopodin protein in HG-exposed podocytes treated with APO or DPI ( = 4–8). Ctrl: control; HG: high glucose; Veh: vehicle; Scram: scrambled shRNA; shgp91: shRNA; APO: apocynin; DPI: diphenyleneiodonium. compared with Ctrl; compared with HG group treated with vehicle or transfected with scramble shRNA.

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