Research Article
Species-Related Differences in the Proteome of Rat and Human Pancreatic Beta Cells
Table 1
Overview of proteome coverage by LC-MS/MS.
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Unfractionated protein extracts of FACS-purified pancreatic beta and alpha cells () and FACS-enriched human islet endocrine cells () were analyzed by alternate-scanning LC-MS/MS. As additional quality control, one pancreatic exocrine cell-enriched human sample was also measured. All samples were injected in triplicate, yielding 372 (300) to 506 (465) proteins that met the criteria for identification (quantification, if detected in of 3 technical replicates) as specified in Methods, with dynamic ranges (difference between the lowest and the highest quantifiable concentration) ranging from 226 to 746 as listed. |