Moderate Intensity Training Impact on the Inflammatory Status and Glycemic Profiles in NOD Mice

Figure 6

(a) Islet of Langerhans visualized by immunofluorescence microscopy. This figure is a representative immunostaining of pancreatic sections, from a NOD diabetic mouse after 6 weeks of exercise. The entire tissue section is captured by a modified method of “virtual slice image capture” using a microscope with a 10x objective. Each virtual slice taken at multiple fluorescent channels is merged into one composite, shown as insulin (green), glucagon (red), and DAPI for the nuclei. Quantitatively, the cell composition of these mouse islets was more than 70%  β-cells and less than 20%  α-cells. Analysis of the immune infiltrates (blue) in the pancreatic islets of diabetic untrained (b) or trained (c) animals at 12th week of exercise (20x objective). (d, e) Analysis of the pancreatic islets of two mice died at week 10 of exercise because of earlier diabetic incidence (occurred at 7th week of training) (20x objective). (f) Whiskers plot of the ratio α-cells/infiltrate in controls and trained mice at the end of the 12 weeks of training.

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