In Vitro and In Vivo Effects of Metformin on Osteopontin Expression in Mice Adipose-Derived Multipotent Stromal Cells and Adipose Tissue

Figure 2

The morphology of adipose-derived mesenchymal stem cells isolated from subcutaneous fat tissue of mice in standard (a), osteogenic (b) and adipogenic culture (c). The calcium deposits formed in extracellular matrix of osteogenic culture were stained using Alizarin Red. The lipid-rich cellular organelles formed after adipogenic stimulation are visible as green droplets. Images included in the graph were chosen as representative. Both control and experimental cultures were carried out in triplicate. Magnification used is 100x. Scale bar is 200 μm.