In Vitro and In Vivo Effects of Metformin on Osteopontin Expression in Mice Adipose-Derived Multipotent Stromal Cells and Adipose Tissue

Figure 3

Proliferation activity of ASCs after metformin treatment at concentrations of 1 mM, 5 mM, and 10 mM in comparison to the control culture (CTRL). The -axis refers to the time of cells’ propagation. The difference between absorbance read at 600 nm and 690 nm, including absorbance of blank sample, was indicated as mark (-axis). The statistical significance () was indicated with asterisk . Error bars represent standard deviation from the mean value calculated from three separated measurements.