Charge-Based Inhibitors of Amylin Fibrillization and Toxicity

Figure 2

Fibrillization kinetics of WT and charged amylin variants. (a) Reaction profiles for 20 M peptide concentrations in the presence of 150 mM NaCl. (b) For the two variants Arg-2 and Mem-T that failed to fibrillize at 20 M peptide concentrations, aggregation was also studied at a larger 400 M peptide concentrations and the indicated salt concentrations. Representative error bars, calculated as the SEM from triplicate measurements, are shown for WT-amylin in (a) and for Mem-T at 300 mM NaCl in (b).

(a)

(b)