Charge-Based Inhibitors of Amylin Fibrillization and Toxicity

Figure 7

Dependence of kinetic parameters for the fibrillization of 80 M WT amylin (see Figure 6) on the concentration of Arg-1 inhibitor. Experiments were done in duplicate in the presence of 150 mM NaCl. Data points are average values for the kinetic parameters, uncertainty bars are SEM values from the duplicate reactions, and curves are four-parameter fits of the inhibition data to the IC₅₀ equation. The IC₅₀ values were  M for the elongation rates (a),  M for the lag times (b), and  M for the steady-state fluorescence plateaus (c).

(a)

(b)

(c)