NADPH Oxidase 4-Derived H₂O₂ Promotes Aberrant Retinal Neovascularization via Activation of VEGF Receptor 2 Pathway in Oxygen-Induced Retinopathy

Figure 1

Nox4 expression in retinas from OIR and control mice at P12. (a) Expression of Nox4 protein in the retina in P12 OIR and control mice was determined by Western blot analysis and semiquantified by densitometry. for each group. (b) Expression of Nox4 mRNA in the retina in P12 OIR and control mice was determined by real-time RT-PCR and normalized to 18 s (left panel) or to CD31 (right panel). for control group and for OIR group. or versus Con. (c) Representative images of retinal whole mounts from P12 control mice stained with anti-Nox4 antibody (green) and anti-CD31 antibody (red). (d) Cryosections of eyeballs from P12 OIR and control mice were stained with anti-Nox4 antibody (green) and anti-CD31 antibody (red).

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