NADPH Oxidase 4-Derived H₂O₂ Promotes Aberrant Retinal Neovascularization via Activation of VEGF Receptor 2 Pathway in Oxygen-Induced Retinopathy

Figure 6

Knockdown of Nox4 ameliorates retinal NV in OIR mice. Ad-Ctrli and Ad-Nox4i were periocularly delivered to OIR mice at P12. (a-b) Retinal expression of Nox4 was determined by Western blot analysis at P15 (a) and immunostaining (b) at P17. for each group; versus Ctrli. (b) Upper panel: Ad-Ctrli injected group and lower panel: Ad-Nox4i injected group. (c-d) Retinal NV formation was evaluated at P17 by counting nuclei of pre-ILM vasculature after HE staining (c) and by retinal angiography (d). HE staining: (c-A) retina from Ad-Ctrli injected mouse, (c-B) retina from Ad-Nox4i injected mouse, and (c-C) quantification result from 6 different mice in each group. Retinal angiography (d-A) retina from Ad-Ctrli injected mouse, (d-B) retina from Ad-Nox4i injected mouse, and (d-C) quantification result from 5 different mice in each group. or versus Ctrli. (e) Phosphorylation of ERK (green) and its colocalization with endothelial marker CD31 (red) were evaluated by immunostaining in P17 retinas. Images were representative of 4 different mice.

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