miRNA-375 a Sensor of Glucotoxicity Is Altered in the Serum of Children with Newly Diagnosed Type 1 Diabetes

Figure 1

Quantification of miR-375 in sera of newly diagnosed T1D children. Data are expressed as Ct values. The Ct is defined as the number of cycles required for the fluorescent signal to cross the threshold (i.e. exceeding background level). Ct levels are inversely proportional to the amount of target nucleic acid in the sample (i.e. the lower the Ct level, the greater the amount of target nucleic acid in the sample). To analyze individual qPCR data, we first controlled the quality of RNA extraction by quantifying Cel-miR-39 (spike). The following formula was used for normalizing the values of miR-375 in all sera samples: raw value − [(spike-in average value of sample) − (median spike-in )]. Data are expressed as mean values ± SEM.