High Glucose-Induced Oxidative Stress Mediates Apoptosis and Extracellular Matrix Metabolic Imbalances Possibly via p38 MAPK Activation in Rat Nucleus Pulposus Cells

Figure 1

Viability and apoptosis of nucleus pulposus cells. (a) Viability evaluation of nucleus pulposus cells cultured with various concentrations of glucose after 12, 24, and 36 hours. (b) Representative sample of flow cytometry analysis of apoptosis using annexin V-FITC and propidium iodide staining. (c) The apoptosis rate of nucleus pulposus cells cultured with various concentrations of glucose. NAC: N-acetylcysteine, SB: SB 202190. Values are expressed as means ± SEM. versus control; versus 25 mM glucose group.

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