High Glucose-Induced Oxidative Stress Mediates Apoptosis and Extracellular Matrix Metabolic Imbalances Possibly via p38 MAPK Activation in Rat Nucleus Pulposus Cells

Figure 2

Effect of high glucose (HG, 25 mM) on reactive oxygen species (ROS) production in nucleus pulposus cells in the absence or presence of N-acetylcysteine (NAC) or SB 202190 (SB). The fluorescence dye 2′,7′-dichlorodihydrofluorescein (DCF) was used to monitor changes in ROS production. Values are expressed as means ± SEM. versus control; versus HG group.