Serotonin- and Dopamine-Related Gene Expression in db/db Mice Islets and in MIN6 β-Cells Treated with Palmitate and Oleate

Figure 3

mRNA expression of energy metabolism-related genes in NEFAs-treated MIN6 β-cells. MIN6 β-cells were treated during twenty-four hours with palmitate or oleate (0.5 mM). Then, total RNA was extracted and the mRNA levels of genes related to glucose and lipid metabolism and with mitochondrial function were evaluated by RT-qPCR. The mRNA levels are expressed as fold change in of the specific gene in the treated versus control conditions (see Section 2). Graph bars represent mean ± standard errors of measurements of three independent experiments in triplicate. The numbers above bars represent the mean Ct value in control cells. The symbol denotes and denotes in one-way ANOVA.