Serotonin- and Dopamine-Related Gene Expression in db/db Mice Islets and in MIN6 β-Cells Treated with Palmitate and Oleate

Figure 4

mRNA expression of monoamines-related genes in NEFAs-treated MIN6 β-cells. MIN6 β-cells were treated during twenty-four hours with palmitate or oleate (0.5 mM). Then, total RNA was extracted and mRNA levels of genes related to the monoamines biosynthesis, degradation, and transport were evaluated by RT-qPCR. The mRNA levels are expressed as fold change between of the specific gene in the treated versus control conditions (see Section 2). Graph bars represent mean ± standard errors of measurements of three independent experiments in triplicate. The numbers above bars represent the mean Ct value in control cells. The symbol denotes and denotes in one-way ANOVA.