Treatment with Tacrolimus and Sirolimus Reveals No Additional Adverse Effects on Human Islets In Vitro Compared to Each Drug Alone but They Are Reduced by Adding Glucocorticoids

Figure 1

The effect of immunosuppressant drugs on human islets function and survival. Human islets treated without or with tacrolimus (TAC 30 µg/L), sirolimus (SRL 30 µg/L), or a combination thereof with or without methylprednisolone (MP 100 ng/L) for 24 h. (a) Glucose-stimulated insulin secretion (GSIS) and (b) corresponding stimulation index (SI) (ratio stimulated to basal glucose-stimulated insulin secretion) in human islets as described in Section 2. (c) Apoptotic cell death measured by the Cell Death Detection in human islet following the same culture conditions. Data are presented as means ± SEM of five separate experiments from five different donors. , versus control.

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