Reversibility of β-Cell-Specific Transcript Factors Expression by Long-Term Caloric Restriction in db/db Mouse

Figure 2

Islet morphology in db/m-F, db/db-F, and db/db-R mice. (a) Representative islet cells from db/m-F, db/db-F, and db/db-R mice stained for insulin (red) and glucagon (Gcg, green). Increased intraislet α-cells were observed in db/db-F mice, while most of the α-cells located in the mantle of islet cells were observed in db/m-F and db/db-R mice. (b) Proportion of Gcg positive cells observed in the islet cells in db/m-F, db/db-F, and db/db-R mice. . db/db-F and db/db mice given free access to regular chow; db/db-R and db/db mice receiving restricted food supply; db/m-F and db/m mice given free access to regular chow. Scale bars: 25 μm.

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