Reversibility of β-Cell-Specific Transcript Factors Expression by Long-Term Caloric Restriction in db/db Mouse

Figure 3

β-cell dedifferentiation in db/m-F, db/db-F, and db/db-R mice. (a) Immunofluorescence analysis of Chromogranin A (ChrA) and insulin expression in db/m-F, db/db-F, and db/db-R mice. (b) ChrA-positive cells with low levels of insulin expression (ChrA⁺) were significantly increased in the db/db-F mice. The arrow indicates the ChrA⁺ cell. (c) Proportion of ChrA⁺ cells observed in the islet cells in db/m-F, db/db-F, and db/db-R mice. . db/db-F and db/db mice given free access to regular chow; db/db-R and db/db mice receiving restricted food supply; db/m-F and db/m mice given free access to regular chow. Scale bars: 25 μm.

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