Reversibility of β-Cell-Specific Transcript Factors Expression by Long-Term Caloric Restriction in db/db Mouse

Figure 5

The expression levels of specific β-cell transcription factors in β-cells from db/m-F, db/db-F, and db/db-R mice. (a) Immunofluorescence analysis of Nkx6.1, Pdx1, MafA, and Foxo1 expression levels in islets from db/m-F, db/db-F, and db/db-R mice. The quantification of the percentage of β-cells containing these transcription factors is shown. (b) Real-time PCR analysis of Nkx6.1, Pdx1, and MafA expression in islets from db/m-F, db/db-F, and db/db-R mice. , , and . The data shown represent three independent experiments. db/db-F and db/db mice given free access to regular chow; db/db-R and db/db mice receiving restricted food supply; db/m-F and db/m mice given free access to regular chow. Scale bars: 25 μm.

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