Research Article
Postnatal Hematopoiesis and Gut Microbiota in NOD Mice Deviate from C57BL/6 Mice
Figure 1
The proportion of CD11b+Gr-1+ splenocytes of newborn NOD mice is high compared to C57BL/6. Flow cytometric analysis of freshly isolated splenocytes from newborn NOD and C57BL/6 mice for the expression of CD11b, Ly6C, and Ly6G. (a) Time course development of the proportion of viable CD11b+ spleen cells in NOD and C57BL/6 mice at PND 1, 2, 4, and 7. Graphs (b), (c), and (d) depict gated Ly6G-Ly6C+ (d), Ly6G+Ly6C− (c), and Ly6G+Ly6C+ (e) double positive cells. Backgating confirmed that all three populations were CD11b+. (e) Time course of the absolute number of viable cells in spleens of NOD and C5BL/6 mice. Data presented as mean ± SEM (–12 spleens). . .
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