Postnatal Hematopoiesis and Gut Microbiota in NOD Mice Deviate from C57BL/6 Mice

Figure 8

Relative gene expression of RegIIIγ analyzed in intestine tissue from NOD and C57BL/6 mice correlates with Gram-stain sections of the same intestines and OPT scanning. Data were normalized to Actb and then to C57BL/6 PND 1, which were defined as value 1. All groups were included in a 2-way ANOVA. . (a) Relative gene expression of Reg3g, analyzed in intestine tissue from NOD and C57BL/6. (b) Relative gene expression of muc2 in intestinal tissue from NOD and C57BL/6. (c) Gram-stain sections of intestine 60x magnification. Arrows indicate presence of bacteria. In sections of NOD intestine (left side) bacteria were present in the lumen but very few and mostly Gram-positive bacteria were attaching to the villi. In contrast a larger amount of both Gram-negative and Gram-positive bacteria was attaching to the surface of the villi in the intestine of C57BL/6 mice. (d) PND 1 mouse intestine autofluorescence image acquired with the OPT system. Digital sections of the PND 1 intestine illustrated in gray-scale demonstrating autofluorescence anatomy data in NOD and C57BL/6. Intestinal sections from C57BL/6 mice displayed more dens villi compared to NOD mice.

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